Pharmacy Practice MCQ, in this article we will solve, Practice MCQ on the topic, solation and preservation methods for pure cultures. Read following article for your reference.
Isolation and preservation methods for pure cultures
Which of the following is NOT an isolation method?
A) Streak Plate Method
B) Pour Plate Method
C) Cryopreservation
D) Spread Plate Method
What is the main purpose of the streak plate method?
A) To count colonies
B) To isolate pure colonies
C) To preserve cultures
D) To grow anaerobic bacteria
Which isolation method involves mixing a microbial sample with molten agar?
A) Streak Plate Method
B) Pour Plate Method
C) Spread Plate Method
D) Roll Tube Method
Which method is suitable for isolating anaerobic bacteria?
A) Streak Plate Method
B) Pour Plate Method
C) Spread Plate Method
D) Roll Tube Method
What is a disadvantage of the spread plate method?
A) Requires skill to avoid contamination
B) Time-consuming
C) Requires precise dilution
D) Exposure to heat may damage microorganisms
Which preservation method involves storing cultures at 0-4°C?
A) Cryopreservation
B) Lyophilization
C) Refrigeration
D) Paraffin Method
What is the main advantage of cryopreservation?
A) Simple and cost-effective
B) Long-term preservation with minimal genetic changes
C) Suitable for short-term storage
D) Does not require specialized equipment
Which preservation method uses a cryoprotectant solution?
A) Periodic Transfer
B) Refrigeration
C) Cryopreservation
D) Lyophilization
What is lyophilization also known as?
A) Freeze-Drying
B) Cryopreservation
C) Refrigeration
D) Periodic Transfer
Which method is suitable for long-term storage at room temperature?
A) Cryopreservation
B) Lyophilization
C) Refrigeration
D) Periodic Transfer
Which preservation method involves covering cultures with sterile liquid paraffin?
A) Cryopreservation
B) Lyophilization
C) Refrigeration
D) Paraffin Method
What is a disadvantage of periodic transfer to fresh media?
A) Requires specialized equipment
B) Risk of contamination
C) Limited storage duration
D) Requires cryoprotectant
Which isolation method is described as time-consuming and may expose microbes to heat shock?
A) Streak Plate Method
B) Pour Plate Method
C) Spread Plate Method
D) Roll Tube Method
Which method is useful for counting colonies and isolating anaerobes?
A) Streak Plate Method
B) Pour Plate Method
C) Spread Plate Method
D) Roll Tube Method
Which preservation method is suitable for anaerobic bacteria?
A) Cryopreservation
B) Lyophilization
C) Refrigeration
D) Paraffin Method
What is a disadvantage of lyophilization?
A) Requires specialized equipment
B) Risk of contamination
C) Limited storage duration
D) Requires periodic transfer
Which isolation method involves spreading a microbial sample on the inner surface of a test tube?
A) Streak Plate Method
B) Pour Plate Method
C) Spread Plate Method
D) Roll Tube Method
Which preservation method involves freezing and then dehydrating cultures under vacuum?
A) Cryopreservation
B) Lyophilization
C) Refrigeration
D) Paraffin Method
What is a disadvantage of the pour plate method?
A) Requires skill to avoid contamination
B) Time-consuming
C) Requires precise dilution
D) Exposure to heat may damage microorganisms
Which method is described as simple and cost-effective for short-term storage?
A) Cryopreservation
B) Lyophilization
C) Refrigeration
D) Periodic Transfer
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