Pharmacy Practice MCQ » PHARMACAREERS

Pharmacy Practice MCQ, in this article we will solve, Practice MCQ under subject Microbiology. Read following article for your reference.

Growth Of Animal Cells In Culture, General Procedure For Cell Culture » PHARMACAREERS

What is the primary purpose of cell culture?
- a) To enhance the flavor of products
- b) To study the behavior of animal cells in a controlled environment
- c) To reduce production costs
- d) To improve aesthetics
Which type of cells are commonly grown in culture for biomedical research?
- a) Bacterial cells
- b) Animal cells
- c) Plant cells
- d) Fungal cells
What is the main component of the culture medium for animal cells?
- a) Water
- b) Agar
- c) Nutrient-rich solution
- d) Glucose
Which of the following is a common step in the preparation of a cell culture?
- a) Autoclaving the culture medium
- b) Sterilizing the workspace
- c) Seeding the cells
- d) All of the above
What is the purpose of using antibiotics in cell culture?
- a) To enhance cell growth
- b) To prevent contamination by bacteria
- c) To improve the color of the culture
- d) To reduce the cost of the culture medium
Which equipment is essential for maintaining sterile conditions in cell culture?
- a) Centrifuge
- b) Laminar flow hood
- c) Microscope
- d) Incubator
What is the role of an incubator in cell culture?
- a) To provide light for photosynthesis
- b) To maintain optimal temperature and humidity for cell growth
- c) To sterilize the culture medium
- d) To measure cell density
Which of the following is a common method for sterilizing culture media?
- a) Filtration
- b) Boiling
- c) Freezing
- d) Drying
Why is it important to use aseptic techniques in cell culture?
- a) To enhance cell growth
- b) To prevent contamination by microorganisms
- c) To reduce the cost of the culture medium
- d) To improve the aesthetics of the culture
What is the purpose of subculturing in cell culture?
- a) To analyze cell metabolism
- b) To expand the cell population
- c) To measure cell density
- d) To sterilize the culture medium
Which of the following is a type of cell culture?
- a) Suspension culture
- b) Monolayer culture
- c) Three-dimensional culture
- d) All of the above
What is the primary advantage of using primary cells in culture?
- a) They are easy to maintain
- b) They closely mimic the behavior of cells in vivo
- c) They are resistant to contamination
- d) They grow rapidly
What is the main disadvantage of using continuous cell lines in culture?
- a) They are difficult to maintain
- b) They do not closely mimic the behavior of cells in vivo
- c) They are prone to contamination
- d) They grow slowly
Which type of culture medium is commonly used for growing mammalian cells?
- a) LB medium
- b) DMEM (Dulbecco’s Modified Eagle Medium)
- c) Agar medium
- d) Nutrient broth
What is the purpose of adding fetal bovine serum (FBS) to the culture medium?
- a) To provide essential growth factors and nutrients
- b) To prevent contamination
- c) To enhance the color of the culture
- d) To reduce the cost of the culture medium
Why is it important to monitor the pH of the culture medium?
- a) To ensure optimal cell growth conditions
- b) To improve the color of the culture
- c) To reduce the cost of the culture medium
- d) To enhance cell proliferation
What is the purpose of trypsinization in cell culture?
- a) To measure cell density
- b) To detach adherent cells from the culture vessel
- c) To sterilize the culture medium
- d) To enhance cell growth
Which equipment is used to count cells in a culture?
- a) Laminar flow hood
- b) Centrifuge
- c) Hemocytometer
- d) Incubator
What is the role of CO2 in the cell culture incubator?
- a) To provide light for photosynthesis
- b) To maintain the pH of the culture medium
- c) To sterilize the culture medium
- d) To enhance cell growth
Why is it important to use a laminar flow hood for cell culture work?
- a) To provide light for photosynthesis
- b) To maintain sterile conditions and prevent contamination
- c) To enhance cell growth
- d) To measure cell density
Which of the following is a common contaminant in cell culture?
- a) Bacteria
- b) Fungi
- c) Mycoplasma
- d) All of the above
What is the purpose of using a CO2 incubator in cell culture?
- a) To provide light for photosynthesis
- b) To maintain optimal temperature, humidity, and CO2 levels for cell growth
- c) To sterilize the culture medium
- d) To measure cell density
Why is it important to regularly monitor cell morphology in culture?
- a) To ensure optimal growth conditions
- b) To detect contamination
- c) To assess cell health and viability
- d) All of the above
Which method is commonly used to measure cell viability in culture?
- a) Microscopy
- b) Trypan blue exclusion assay
- c) Spectrophotometry
- d) Flow cytometry
What is the significance of using serum-free media in cell culture?
- a) To enhance cell growth
- b) To provide a defined and consistent culture environment
- c) To reduce the cost of the culture medium
- d) To improve the color of the culture
What is the purpose of adding antibiotics to the culture medium?
- a) To enhance cell growth
- b) To prevent bacterial contamination
- c) To improve the color of the culture
- d) To reduce the cost of the culture medium
Which type of cells are commonly used for vaccine production?
- a) Bacterial cells
- b) Animal cells
- c) Plant cells
- d) Fungal cells
What is the role of serum in the culture medium?
- a) To provide essential growth factors and nutrients
- b) To enhance the color of the culture
- c) To prevent contamination
- d) To reduce the cost of the culture medium
Which of the following is a method for preserving cells for long-term storage?
- a) Drying
- b) Freezing in liquid nitrogen
- c) Boiling
- d) Autoclaving
Why is it important to use aseptic techniques when handling cell cultures?
- a) To enhance cell growth
- b) To prevent contamination
- c) To improve the color of the culture
- d) To reduce the cost of the culture medium

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