Pharmacy Practice MCQ » PHARMACAREERS

Pharmacy Practice MCQ, in this article we will solve, Practice MCQ under subject Microbiology. Read following article for your reference.

Phase Contrast Microscopy, Dark Field Microscopy And Electron Microscopy » PHARMACAREERS

Who invented phase contrast microscopy?
- A) Albert Einstein
- B) Frits Zernike
- C) Robert Hooke
- D) Antonie van Leeuwenhoek
What is the primary advantage of phase contrast microscopy?
- A) High magnification
- B) Observing live cells without staining
- C) High resolution
- D) Low cost
What type of light source is typically used in phase contrast microscopy?
- A) LED
- B) Fluorescent
- C) Tungsten-halogen lamp
- D) Laser
What does the phase plate in phase contrast microscopy do?
- A) Magnifies the image
- B) Converts phase shifts into brightness changes
- C) Stains the specimen
- D) Filters the light
In positive phase contrast, how does the specimen appear?
- A) Brighter against a dark background
- B) Darker against a bright background
- C) Colorful
- D) Transparent

What is the main feature of dark field microscopy?
- A) Uses electrons instead of light
- B) Makes tiny, clear objects stand out against a dark background
- C) Requires staining of specimens
- D) Uses polarized light
Which of the following is a key application of dark field microscopy?
- A) Observing stained specimens
- B) Viewing live, unstained cells
- C) Studying fluorescent samples
- D) Imaging metallic surfaces
What type of condenser is used in dark field microscopy?
- A) Bright field condenser
- B) Phase contrast condenser
- C) Dark field condenser
- D) Fluorescent condenser
What is the primary advantage of dark field microscopy?
- A) High resolution
- B) High contrast for transparent specimens
- C) Low cost
- D) Easy to use
In dark field microscopy, how does the specimen appear?
- A) Dark against a bright background
- B) Bright against a dark background
- C) Colorful
- D) Transparent

What is the main difference between electron microscopy and light microscopy?
- A) Electron microscopy uses light
- B) Electron microscopy uses electrons
- C) Electron microscopy uses sound waves
- D) Electron microscopy uses magnetic fields
Which type of electron microscopy provides detailed surface images?
- A) Transmission Electron Microscopy (TEM)
- B) Scanning Electron Microscopy (SEM)
- C) Phase Contrast Microscopy
- D) Dark Field Microscopy
What is the primary advantage of electron microscopy?
- A) Observing live cells
- B) High resolution and detailed images
- C) Low cost
- D) Easy to use
What is used as the source of illumination in electron microscopy?
- A) Light
- B) Electrons
- C) X-rays
- D) Gamma rays
Which of the following is a limitation of electron microscopy?
- A) Low resolution
- B) Cannot observe live specimens
- C) Requires staining
- D) Low magnification

Which microscopy technique is best for observing live, unstained cells?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Fluorescence Microscopy
Which microscopy technique uses a phase plate?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Bright Field Microscopy
Which microscopy technique is known for providing high contrast images of transparent specimens?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Polarized Light Microscopy
Which microscopy technique uses electrons instead of light to form images?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Fluorescence Microscopy
Which microscopy technique is most suitable for studying the internal structure of cells?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Transmission Electron Microscopy (TEM)
- D) Scanning Electron Microscopy (SEM)
Which microscopy technique enhances the contrast of transparent specimens by converting phase shifts into brightness changes?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Fluorescence Microscopy
Which microscopy technique is commonly used to observe bacteria and other microorganisms?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Bright Field Microscopy
Which microscopy technique requires a special condenser to create a dark background?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Fluorescence Microscopy
Which microscopy technique can achieve the highest magnification and resolution?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Bright Field Microscopy
Which microscopy technique is best for observing the surface details of a specimen?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Scanning Electron Microscopy (SEM)
- D) Transmission Electron Microscopy (TEM)
Which microscopy technique uses a tungsten-halogen lamp as a light source?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Fluorescence Microscopy
Which microscopy technique is used to observe the fine details of cell organelles?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Transmission Electron Microscopy (TEM)
- D) Scanning Electron Microscopy (SEM)
Which microscopy technique is best for observing unstained, transparent specimens?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Fluorescence Microscopy
Which microscopy technique uses a phase annulus in the condenser?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Electron Microscopy
- D) Bright Field Microscopy
Which microscopy technique is used to observe the detailed surface structure of materials?
- A) Phase Contrast Microscopy
- B) Dark Field Microscopy
- C) Scanning Electron Microscopy (SEM)
- D) Transmission Electron Microscopy (TEM)

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