Pharmacy Practice MCQ, in this article we will solve, Practice MCQ under subject Microbiology. Read following article for your reference.
Principles And Methods Of Different Microbiological Assays » PHARMACAREERS
- What is the primary purpose of microbiological assays in pharmaceutical industries?
- a) To determine the pH of a solution
- b) To evaluate the potency and concentration of antibiotics
- c) To measure the density of a liquid
- d) To calculate the melting point of a substance
- Which of the following is a commonly used method for antibiotic potency testing?
- a) Titration
- b) Gravimetric analysis
- c) Agar diffusion method
- d) Spectroscopy
- What is the principle behind the agar diffusion method?
- a) Measurement of light absorption
- b) Calculation of solubility
- c) Diffusion of antibiotic through an agar medium to inhibit bacterial growth
- d) Separation of particles based on density
- Which type of microorganism is commonly used in antibiotic assays?
- a) Fungi
- b) Bacteria
- c) Algae
- d) Protozoa
- In an agar diffusion test, what is measured to determine the potency of an antibiotic?
- a) Absorbance of light
- b) Diameter of the inhibition zone
- c) Weight of the sample
- d) Volume of the solution
- What is the broth dilution method used for?
- a) Determining the color of a solution
- b) Estimating the solubility of a compound
- c) Measuring the minimum inhibitory concentration (MIC) of an antibiotic
- d) Calculating the boiling point of a liquid
- Which medium is commonly used in the broth dilution method?
- a) Nutrient agar
- b) MacConkey agar
- c) Mueller-Hinton broth
- d) Blood agar
- What does MIC stand for in microbiological assays?
- a) Minimum inhibitory concentration
- b) Maximum inhibition capacity
- c) Medium isolation component
- d) Microbial infection count
- Which of the following assays is used to test the efficacy of disinfectants?
- a) Agar diffusion test
- b) Broth dilution method
- c) Use-dilution test
- d) Spectrophotometric assay
- In microbiological assays, what is the role of control samples?
- a) To provide aesthetic value
- b) To serve as a reference for comparison
- c) To increase the concentration of the solution
- d) To alter the pH of the medium
- Which assay method involves the use of a spectrophotometer to measure microbial growth?
- a) Agar diffusion method
- b) Use-dilution test
- c) Optical density measurement
- d) Broth dilution method
- What is the principle behind the use-dilution test for disinfectants?
- a) Measuring light absorption
- b) Calculating solubility
- c) Evaluating the effectiveness of disinfectants on contaminated surfaces
- d) Measuring density
- What is the purpose of a positive control in microbiological assays?
- a) To ensure no microbial growth
- b) To validate the test method
- c) To alter the pH of the medium
- d) To reduce the concentration of the solution
- Which of the following is an indicator organism commonly used in sterility testing?
- a) Escherichia coli
- b) Staphylococcus aureus
- c) Bacillus subtilis
- d) Clostridium sporogenes
- In microbiological assays, what is the significance of the zone of inhibition?
- a) Indicates the presence of fungal contamination
- b) Measures the density of the medium
- c) Represents the area where microbial growth is inhibited
- d) Determines the solubility of the compound
- Which microbiological assay method is used to determine the antimicrobial activity of a sample by measuring the light transmittance?
- a) Agar diffusion method
- b) Optical density measurement
- c) Broth dilution method
- d) Use-dilution test
- What is the main advantage of using the agar diffusion method in microbiological assays?
- a) It is a time-consuming method
- b) It provides quantitative results
- c) It requires expensive equipment
- d) It is easy to set up and interpret
- What is the key component measured in a broth dilution assay?
- a) Solubility of the compound
- b) Weight of the sample
- c) Growth inhibition of microorganisms
- d) Color change in the medium
- Which of the following is a method for evaluating the effectiveness of antiseptics?
- a) Spectrophotometric assay
- b) Use-dilution test
- c) Gravimetric analysis
- d) Titration
- What is the purpose of using a neutralizer in disinfectant efficacy testing?
- a) To increase microbial growth
- b) To neutralize the effect of the disinfectant
- c) To alter the pH of the medium
- d) To enhance the solubility of the compound
- In microbiological assays, what is the role of a negative control?
- a) To serve as a reference for comparison
- b) To ensure no microbial contamination
- c) To validate the test method
- d) To increase the concentration of the solution
- Which of the following assays is used to determine the bactericidal activity of an antibiotic?
- a) Use-dilution test
- b) Broth microdilution test
- c) Agar diffusion method
- d) Spectrophotometric assay
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