Pharmacy Practice MCQ » PHARMACAREERS

Pharmacy Practice MCQ, in this article we will solve, Practice MCQ under subject Microbiology. Read following article for your reference.

Primary, Established And Transformed Cell Culture » PHARMACAREERS

What is the primary characteristic of primary cell cultures?
- a) They are derived directly from tissues
- b) They are immortal
- c) They do not require growth factors
- d) They have high mutation rates
Which type of cell culture is known for its limited lifespan?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is a common method for establishing primary cell cultures?
- a) Transfection
- b) Cell fusion
- c) Tissue dissociation
- d) Radiation
What defines an established cell line?
- a) It is derived from stem cells
- b) It can proliferate indefinitely
- c) It is resistant to antibiotics
- d) It is derived from bone marrow
Which of the following is an example of an established cell line?
- a) HeLa cells
- b) Fibroblasts from human skin
- c) Primary hepatocytes
- d) Freshly isolated neurons
What is the main characteristic of transformed cell lines?
- a) They can proliferate indefinitely and have undergone genetic changes
- b) They are derived from primary cell cultures
- c) They do not adhere to surfaces
- d) They require specialized growth media
Which type of cell culture is often used in cancer research due to its rapid growth and genetic mutations?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is the purpose of using trypsin in cell culture?
- a) To sterilize the medium
- b) To detach adherent cells from the culture vessel
- c) To induce cell differentiation
- d) To enhance cell proliferation
Which cell culture is most commonly used for vaccine production?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is a significant advantage of using primary cell cultures in research?
- a) High proliferation rate
- b) Genetic stability
- c) Unlimited lifespan
- d) Resistance to contamination
Which type of cell line has undergone immortalization?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is a common application of established cell lines in research?
- a) Studying drug metabolism
- b) Investigating genetic mutations
- c) Producing monoclonal antibodies
- d) All of the above
Which type of cell culture requires the most stringent sterile techniques to prevent contamination?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is a common method used to immortalize primary cells to create an established cell line?
- a) Electroporation
- b) Transfection with oncogenes
- c) Chemical treatment
- d) Cryopreservation
Which type of cell culture is often used to study cellular differentiation and development?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
Which cell culture is most likely to mimic the in vivo environment?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is a disadvantage of using transformed cell lines in research?
- a) Genetic instability
- b) Limited lifespan
- c) Low proliferation rate
- d) High cost
Which type of cell line can be used for long-term storage and later revived for experiments?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is the purpose of using serum in cell culture media?
- a) To provide growth factors and nutrients
- b) To enhance cell adhesion
- c) To increase pH
- d) To sterilize the medium
Which cell culture technique involves fusing two different cell types to create a hybrid cell line?
- a) Primary culture
- b) Transfection
- c) Hybridoma technology
- d) Electroporation
What is a common contaminant that researchers must be cautious of in cell culture?
- a) Mycoplasma
- b) Fungus
- c) Bacteria
- d) All of the above
Which type of cell line is most suitable for high-throughput drug screening?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is the benefit of using cryopreservation for cell lines?
- a) Immediate cell proliferation
- b) Long-term storage without genetic changes
- c) Enhanced cell differentiation
- d) Improved cell adhesion
Which type of cell line is often used to study viral infections and vaccine development?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is a key consideration when choosing between primary and established cell lines for an experiment?
- a) Cost of media
- b) Relevance to the study’s objectives
- c) Availability of equipment
- d) Incubation temperature
Which method is commonly used to validate the identity of an established cell line?
- a) DNA fingerprinting
- b) Microscopy
- c) Flow cytometry
- d) Spectrophotometry
Which type of cell culture is often used for the production of monoclonal antibodies?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is a potential drawback of using primary cell cultures in long-term studies?
- a) High proliferation rate
- b) Genetic instability
- c) Limited lifespan
- d) Resistance to antibiotics
Which type of cell culture is often used to study the effects of cancer treatments?
- a) Primary cell culture
- b) Established cell line
- c) Transformed cell line
- d) Hybridoma cell line
What is the importance of maintaining aseptic conditions in cell culture?
- a) To enhance cell growth
- b) To prevent contamination and ensure reproducibility
- c) To improve cell adhesion
- d) To increase the cost of the culture medium

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